Journal
BLOOD
Volume 114, Issue 24, Pages 4960-4967Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2009-04-218156
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Funding
- Deutsche Forschungsgemeinschaft [WE817/6, SFB 621-A5]
- Deutsche Krebshilfe
- Ministry for Education and Research (Bundesministerium fur Bildung, Wissenschaft, Forschung und Technologie)
- Hannover Biomedical Research School
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To study B-cell development from bone marrow (BM), we generated recombination-activating gene 1 (Rag1)-targeted mice lacking mature lymphocytes. B-cell development can be induced in such mice by B cell-specific restoration of a functional Rag1 transcription unit. Follicular and marginal zone B cells populated the spleen when Rag1 expression was permitted. Notably, the peritoneal cavity was dominated by bona fide B-1a cells, as judged by surface markers and functional properties. These BM-derived B-1a cells exhibited a polyclonal VDJ repertoire with substantial N nucleotide insertions. Nevertheless, physiologic frequencies of phosphatidylcholine-specific B cells were detected. Importantly, the BM of young and 5-month-old mice was indistinguishable with regard to the potential to generate B-1a cells. (Blood. 2009; 114: 4960-4967)
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