Journal
BLOOD
Volume 113, Issue 21, Pages 5144-5156Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2008-10-185751
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Funding
- Medical Research Council (MRC, London, United Kingdom)
- Biotechnology and Biological Sciences Research Council (BBSRC, Swindon, United Kingdom)
- Wellcome Trust (London, United Kingdom)
- Leukaemia Research Foundation (LRF, London, United Kingdom)
- Foulkes Foundation Fellowship
- Biotechnology and Biological Sciences Research Council [BB/D522770/1] Funding Source: researchfish
- Medical Research Council [G0000121] Funding Source: researchfish
- MRC [G0000121] Funding Source: UKRI
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Hedgehog signaling regulates differentiation, survival, and proliferation of the earliest double-negative (DN) thymocytes, but its importance at later stages of T-cell development is controversial. Here we use loss- and gain-of-function mouse models to show that Shh, by signaling directly to the developing thymocyte, is a negative regulator of pre-TCR-induced differentiation from DN to double-positive (DP) cell. When hedgehog signaling was reduced, in the Shh(-/-) and Gli2(-/-) thymus, or by T lineage-specific transgenic expression of a transcriptional-repressor form of Gli2 (Gli2 Delta C-2), differentiation to DP cell after pre-TCR signal transduction was increased. In contrast, when Hh signaling was constitutively activated in thymocytes, by transgenic expression of a constitutive transcriptional-activator form of Gli2 (Gli2 Delta N-2), the production of DP cells was decreased. Gene expression profiling showed that physiologic Hh signaling in thymocytes maintains expression of the transcription factor FoxA2 on pre-TCR signal transduction. (Blood. 2009; 113: 5144-5156)
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