Journal
BLOOD
Volume 115, Issue 8, Pages 1549-1553Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2009-05-219907
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Funding
- Program Hospitalier de Recherche Clinique [00166]
- Agence de la Biomedecine
- Ligue Contre le Cancer
- Canceropole Grand Ouest
- Etablissement Francais du Sang
- European Commission: Cultivated Adult Stem Cells as Alternative for Damaged Tissue [223236, HEALTH-F5-2009-223236]
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Clinical-grade human mesenchymal stromal cells (MSCs) have been expanded in vitro for tissue engineering or immunoregulatory purposes without standardized culture conditions or release criteria. Although human MSCs show poor susceptibility for oncogenic transformation, 2 recent studies described their capacity to accumulate chromosomal instability and to give rise to carcinoma in immunocompromised mice after long-term culture. We thus investigated the immunologic and genetic features of MSCs expanded with fetal calf serum and fibroblast growth factor or with platelet lysate in 4 cell-therapy facilities during 2 multicenter clinical trials. Cultured MSCs showed a moderate expression of human leukocyte antigen-DR without alteration of their low immunogenicity or their immunomodulatory capacity. Moreover, some transient and donor-dependent recurring aneuploidy was detected in vitro, independently of the culture process. However, MSCs with or without chromosomal alterations showed progressive growth arrest and entered senescence without evidence of transformation either in vitro or in vivo. (Blood. 2010;115:1549-1553)
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