Journal
BLOOD
Volume 113, Issue 5, Pages 1006-1015Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2008-05-156059
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Funding
- Italian Ministry of Health
- Italian Ministry of Research and University
- Fondazione Cariplo
- Italian Association for Cancer Research
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Long-term clinical remissions of leukemia, after allogeneic hematopoietic stem cell transplantation, depend on alloreactive memory T cells able to self-renew and differentiate into antileukemia effectors. This is counterbalanced by detrimental graft-versus-host disease (GVHD). Induction of a selective suicide in donor T cells is a current gene therapy approach to abrogate GVHD. Unfortunately, genetic modification reduces alloreactivity of lymphocytes. This associates with an effector memory (T-EM) phenotype of gene-modified lymphocytes and may limit antileukemia effect. We hypothesized that alloreactivity of gene-modified lymphocytes segregates with the central memory (T-CM) phenotype. To this, we generated suicide gene-modified T-CM lymphocytes with a retroviral vector after CD28 costimulation and culture with IL-2, IL-7, or a combination of IL-7 and IL-15. In vitro, suicide gene-modified T-CM cells self-renewed upon alloantigen stimulation and resisted activation-induced cell death. In a humanized mouse model, only suicide gene -modified T cells cultured with IL-7 and IL-15 persisted, differentiated in T-EM cells, and were as potent as unmanipulated lymphocytes in causing GVHD. GVHD was halted through the activation of the suicide gene machinery. These results warrant the use of suicide gene modified T-CM cells cultured with IL-7 and IL-15 for the safe exploitation of the alloreactive response against cancer. (Blood. 2009;113:1006-1015)
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