Journal
BLOOD
Volume 112, Issue 5, Pages 1863-1871Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2008-02-138925
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Funding
- National Institutes of Health (NIH
- Bethesda, MD) [AI28847, AI49993, CA099997]
- Department of Veterans' Affairs
- NIH [AR052125, A1063274, T32 AI007533]
- Lupus Foundation of Minnesota (Bloomington, MN)
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CD40 signaling is critical for innate and adaptive immunity against pathogens, and the cytoplasmic domain of CD40 is highly conserved both within and between species. A novel missense single nucleotide polymorphism (SNP) in the cytoplasmic domain of CD40 at position 227 (P227A) was identified, which resides on a conserved ancestral haplotype highly enriched in persons of Mexican and South American descent. Functional studies indicated that signaling via human (h) CD40-P227A stably expressed in several B-cell lines led to increased phosphorylation of c-Jun, increased secretion of the pro-inflammatory cytokines interleukin (IL)-6 and TNF-alpha, and increased Ig production, compared with wild-type hCD40. Cooperation between hCD40-P227A signaling and B-cell receptor (BCR)- or Toll-like receptor 9 (TLR9)-mediated signaling was also enhanced, resulting in elevated and synergistic production of IL-6 and Ig. We have thus identified a novel genetic variant of hCD40 with a gain-of-function immune phenotype.
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