Journal
BJU INTERNATIONAL
Volume 108, Issue 9, Pages 1421-1429Publisher
WILEY
DOI: 10.1111/j.1464-410X.2011.10169.x
Keywords
robotic prostatectomy; multiphoton microscopy; prostate cancer; nerve sparing; histopathology; real-time imaging
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Funding
- Intuitive Surgical, Inc. (Sunnyvale, CA, USA)
- Prostate Cancer Foundation
- National Institute of Bioimaging and Bioengineering [R01 EB009388-01]
- NIH, NCI [R01 CA116583]
- NIH, NIBIB [P41 RR04224, 1 R01 EB006736]
- Clinical and Translational Science Center (CTSC) of the Weill Cornell Medical College [1 KL2 RR024997-01]
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OBJECTIVE To test whether multiphoton microscopy (MPM) might allow identification of prostatic and periprostatic structures with magnification and resolution similar to gold standard histopathology. MATERIAL AND METHODS The present study included 95 robotic radical prostatectomy patients who consented to participate in an Institutional Review Board-approved study starting in 2007. The types of specimens used for imaging were excised surgical margins and biopsies, and sections obtained from the excised prostate. The specimens were imaged with a custom-built MPM system. All images were compared with haematoxylin/eosin histopathology of the same specimen. RESULTS MPM of freshly excised, unprocessed and unstained tissue can identify all relevant prostatic and periprostatic structures, such as nerves, blood vessels, capsule, underlying acini and also pathological changes, including prostate cancer. Histological confirmation and correlation of these structures and pathologies have validated the findings of MPM. CONCLUSIONS MPM shows great promise as a tool for real- time intra-surgical histopathology without needing excision or administration of contrast agents. The results will, however, need to be confirmed in true surgical settings using a miniaturized MPM microendoscope.
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