Journal
BJU INTERNATIONAL
Volume 108, Issue 4, Pages 566-570Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1464-410X.2010.09888.x
Keywords
antioxidants; high-density lipoproteins; male subfertility; paraoxonase 1; paraoxonase 2; paraoxonase 3
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Funding
- Instituto de Salud Carlos III [FIS 05/1607, 08/1175]
- Ministerio de Sanidad, Madrid, Spain
- Department of Medicine and Surgery of the Universitat Rovira i Virgili, Reus, Spain
- Generalitat de Catalunya [FI08/00064, FI06/01054]
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OBJECTIVE To characterise the immunohistochemical sites of paraoxonase (PON) 1, PON2 and PON3 in human testicular tissue, and to analyse PON1 levels in semen, aiming to investigate the role played by these enzymes in the pathophysiology of male subfertility. PATIENTS AND METHODS The present study was performed in 41 semen samples from normal donors and in 52 semen samples and ten testicle biopsies from patients who were being evaluated for causes of subfertility. RESULTS Immunohistochemical analyses showed high levels of PON1 and PON3 expression in testicular tissue. PON2 expression was also detected, albeit at weaker levels. Oxidative stress indicators in biopsies were low and localized in some specific areas of the seminiferous tubules. PON1 was detected in seminal fluid at very low levels but with no significant differences between patients and controls. Receiver-operating characteristic analysis showed a low diagnostic power of semen PON1 levels. CONCLUSIONS The present study shows high protein expression levels of PON1, PON2 and PON3 in testicular cells. The concentrations and activities of PON1 in semen are negligible and are probably the result of cellular catabolism, with no significant biological function in the testes.
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