Electrochemical oxidation of provitamin B-5, D-panthenol and its analysis in spiked human urine

Background: The behavior of biomolecules and the advancements in the electrochemical techniques play a tremendous role in the development of voltammetric sensors. Redox reactions of biologically active molecules can be studied by using different voltammetry techniques which guide us to understand the metabolic fact of the targeted drug. In the present work, we describe the electrochemical oxidation of D-panthenol (DP) by using a versatile glassy carbon electrode (GCE). Methods: Experimental section was carried out by using cyclic voltammetry and square wave voltammetry. Results: Under the optimized conditions (pH 4.2), the square wave voltammetric peak current of D-panthenol increased linearly with its concentration. The detection limit was found to be 5.0 x 10(-7) M. The number of protons and electrons involved in the oxidation process were calculated. The heterogeneous rate constant was found to be (3.67 x 103 s(-1)). Conclusions: The method detects the trace level of the analyte with low detection limit which imparts the development of a sensor for selective and sensitive determination of D-panthenol. This sensor was successfully applied to determine the D-panthenol in spiked urine samples.