Journal
BJU INTERNATIONAL
Volume 107, Issue 12, Pages 1982-1987Publisher
WILEY
DOI: 10.1111/j.1464-410X.2010.09797.x
Keywords
electroporation; ablation; renal cell carcinoma; laparoscopy; percutaneous; animal model
Categories
Ask authors/readers for more resources
IRE has been used in liver and prostate models before, confirming appropriate cell death with sharp margins of ablation. The current study confirms that this technology may also provide an effective treatment in the kidney, which provides a unique environment due to electrolyte differences, renal blood flow, and collecting system anatomy. The advantage of IRE may be that its athermal nature limits common problems with percutaneous ablation, including heat sink and destruction of the urothelium. OBJECTIVE center dot To evaluate the effects of irreversible electroporation (IRE) on renal parenchyma and the renal collecting system in a porcine model. MATERIALS AND METHODS center dot Eight female Yorkshire pigs underwent a series of laparoscopic ablations using either monopolar or bipolar IRE (Angiodynamics, Queensbury, NY, USA). center dot The pigs were killed between 10 min and 14 days after IRE, and the kidneys were harvested for gross and histological analysis, including NADH staining for cellular viability. RESULTS center dot In all, 24 ablations were performed and all the pigs survived without complications. center dot Initial gross lesions were diffusely haemorrhagic, decreasing progressively in size (30-40%) to small white scars over the 14-day period. center dot Immediately after IRE, ablated tissue was characterized by diffuse tubular desquamation, eosinophilia, and nuclear pyknosis, with absence of cellular viability by NADH. center dot At 7 days after IRE, there was diffuse cellular necrosis with early peripheral granulation changes, and by 14 days there was marked tissue granulation, chronic inflammation, and dystrophic calcification with early fibrosis and cellular contraction. center dot Initial patchy urothelial injury and ulceration showed signs of repair and viability by 14 days after IRE. CONCLUSIONS center dot Renal IRE in the porcine kidney leads to predictable histological changes characteristic of cellular death within 1 h of ablation, with relative urothelial sparing. center dot Further animal studies are warranted to determine safety and efficacy of this novel ablation technology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available