Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 81, Issue 10, Pages 2009-2017Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2017.1359486
Keywords
gut microbes; standard medium; short-chain fatty acids; dominant human gut bacteria; Gifu anaerobic medium
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Funding
- Institute for Fermentation (Osaka, Japan)
- Mitani Foundation for Research and Development
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Recently, a human gut microbial gene catalogue, which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the human gut microbial gene catalogue were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.
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