4.5 Article

Exploring the Potential of Airyscan Microscopy for Live Cell Imaging

Journal

PHOTONICS
Volume 4, Issue 3, Pages -

Publisher

MDPI
DOI: 10.3390/photonics4030041

Keywords

Airyscan; microscopy; confocal and super-resolution microscopy; signal-to-noise-ratio

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Funding

  1. Wellcome Trust [104924/14/Z/14]
  2. Medical Research Council [MC_UU_12010/Unit Programmes G0902418, MC_UU_12025]
  3. University of Oxford
  4. Kennedy Trust for Rheumatology Research [100262/Z/12/Z]

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Biological research increasingly demands the use of non-invasive and ultra-sensitive imaging techniques. The Airyscan technology was recently developed to bridge the gap between conventional confocal and super-resolution microscopy. This technique combines confocal imaging with a 0.2 Airy Unit pinhole, deconvolution and the pixel-reassignment principle in order to enhance both the spatial resolution and signal-to-noise-ratio without increasing the excitation power and acquisition time. Here, we present a detailed study evaluating the performance of Airyscan as compared to confocal microscopy by imaging a variety of reference samples and biological specimens with different acquisition and processing parameters. We found that the processed Airyscan images at default deconvolution settings have a spatial resolution similar to that of conventional confocal imaging with a pinhole setting of 0.2 Airy Units, but with a significantly improved signal-to-noise-ratio. Further gains in the spatial resolution could be achieved by the use of enhanced deconvolution filter settings, but at a steady loss in the signal-to-noise ratio, which at more extreme settings resulted in significant data loss and image distortion.

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