Journal
BIOTECHNOLOGY PROGRESS
Volume 26, Issue 4, Pages 907-918Publisher
WILEY
DOI: 10.1002/btpr.406
Keywords
biomimetic leaf; solar energy; thin-film photobioreactor; hydrogen production; Rhodopseudomonas palustris; microbial photosynthesis; nongrowing
Funding
- University of Minnesota [S03-2004, S03b-2004, LG H4]
- NIGMS Biotechnology [5T32-GM008347]
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Intact cells are the most stable form of nature's photosynthetic machinery. Coating-immobilized microbes have the potential to revolutionize the design of photoabsorbers for conversion of sunlight into fuels. Multi-layer adhesive polymer coatings could spatially combine photoreactive bacteria and algae (complementary biological irradiance spectra) creating high surface area, thin, flexible structures optimized for light trapping, and production of hydrogen (H-2) from water, lignin, pollutants, or waste organics. We report a model coating system which produced 2.08 +/- 0.01 mmol H-2 m(-2) h(-1) for 4,000 h with nongrowing Rhodopseudomonas palustris, a purple nonsulfur photosynthetic bacterium. This adhesive, flexible, nanoporous Rps. palustris latex coating produced 8.24 +/- 0.03 mol H-2 m(-2) in an argon atmosphere when supplied with acetate and light. A simple low-pressure hydrogen production and trapping system was tested using a 100 cm(2) coating. Rps. palustris CGA009 was combined in a bilayer coating with a carotenoid-less mutant of Rps. palustris (Crtl(-)) deficient in peripheral light harvesting (LH2) function. Cryogenic field emission gun scanning electron microscopy (cryo-FEG-SEM) and high-pressure freezing were used to visualize the microstructure of hydrated coatings. A light interaction and reactivity model was evaluated to predict optimal coating thickness for light absorption using the Kubelka-Munk theory (KMT) of reflectance and absorptance. A two-flux model predicted light saturation thickness with good agreement to observed H-2 evolution rate. A combined materials and modeling approach could be used for guiding cellular engineering of light trapping and reactivity to enhance overall photosynthetic efficiency per meter square of sunlight incident on photocatalysts. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 907-918, 2010
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