Journal
BIOTECHNOLOGY LETTERS
Volume 36, Issue 9, Pages 1827-1834Publisher
SPRINGER
DOI: 10.1007/s10529-014-1546-x
Keywords
Arachidonic acid; Fatty acid desaturation; Fatty acid synthesis; Mitochondrial malic enzyme; Mortierella alpina
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Funding
- National Natural Science Foundation of China [21276108, 31271812]
- Program for New Century Excellent Talents [NCET-13-0831]
- National High Technology Research and Development Program of China [2011ARA100905, 2012ARA022105C]
- National Science Fund for Distinguished Young Scholars [31125021]
- National Basic Research Program 973 of China [2012CB720802]
- 111 project [B07029]
- Fundamental Research Funds for the Central Universities [JUSRP51320B]
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Malic enzyme (ME) catalyses the oxidative decarboxylation of l-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6) content increased by 60 % without affecting the total fatty acid content. Our results suggest that enhancing mME activity may be an effective mean to increase industrial production of ARA in M. alpina.
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