Journal
BIOTECHNOLOGY LETTERS
Volume 36, Issue 8, Pages 1701-1709Publisher
SPRINGER
DOI: 10.1007/s10529-014-1531-4
Keywords
Cellulase; Mangrove; Metagenomic library; Organic solvent-resistant; Salt-tolerant
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Funding
- National Basic Research Program of China (973 Program) [2010CB 833801]
- National Natural Science Foundation of China [41230962]
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A novel endoglucanase gene, mgcel44, was isolated from a mangrove soil metagenomic library by functional-based screening. It encodes a 648-aa peptide with a catalytic domain of glycosyl hydrolase family 44. The deduced amino acid sequence of mgcel44 shares less than 50 % identity with endoglucanases in GenBank database. mgcel44 was cloned and overexpressed in Escherichia coli. The recombinant enzyme, MgCel44, has a molecular mass of 70.8 kDa as determined by SDS-PAGE. Its optimal pH and temperature for activity were 6 and 45 A degrees C, respectively. It was highly active at 25-45 A degrees C and pH 5-8. Its activity was enhanced in 0.5 M NaCl by > 1.6-fold and stable up to 1.5 M NaCl. MgCel44 was resistant to several organic solvents and had high activity at 15 % (v/v) solvent after incubating for 24 h at 25 A degrees C.
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