Journal
BIOTECHNOLOGY LETTERS
Volume 37, Issue 5, Pages 1031-1036Publisher
SPRINGER
DOI: 10.1007/s10529-014-1759-z
Keywords
Biofuel; Cellulosic ethanol; Co-utilization of xylose and glucose; Ethanol; D-Ribulose-5-phosphate3-epimerase; Synthetic biology; Xylose fermentation
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Funding
- National Natural Science Foundation of China [21390203]
- Ministry of Science and Technology of China (973 Program) [2013CB733600]
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Simultaneous co-utilization of xylose and glucose is a key issue in engineering microbes for cellulosic ethanol production. We coupled xylose utilization with glucose metabolism by deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) through pentose phosphate pathway flux. Simultaneous utilization of xylose and glucose then occurred in the engineered Saccharomyces cerevisiae strain with the xylose utilization pathway. Xylose consumption occurred at the beginning of glucose consumption by the engineered yeast without RPE1 in a mixed sugar fermentation. About 3.2 g xylose l(-1) was utilized simultaneously with consumption of 40.2 g glucose l(-1) under O-2-limited conditions. In addition, an approximate ratio (similar to 1:10) for xylose and glucose consumption was observed in the fermentation with different sugar concentration by the engineered strain without RPE1. Simultaneous utilization of xylose is realized by the coupling of glucose metabolism and xylose utilization through RPE1 deletion in xylose-utilizing S. cerevisiae.
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