Journal
BIOTECHNOLOGY LETTERS
Volume 36, Issue 5, Pages 975-983Publisher
SPRINGER
DOI: 10.1007/s10529-013-1434-9
Keywords
Bile salts; Bile salt hydrolase; Glycodeoxycholic acid; Hypercholesterolemia; Lactobacillus plantarum; Phylogenetic tree; Taurodeoxycholic acid
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Funding
- 863 (Hi-tech research and development program of China) [2012AA022108, 2008AA10Z336]
- Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China [IRT1166]
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Genes coding for bile salt hydrolase of Lactobacillus plantarum CGMCC 8198, a novel probiotic strain isolated from silage, were identified, analyzed and cloned. L. plantarum strongly resisted the inhibitory effects of bile salts and also decreased serum cholesterol levels by 20 % in mice with hypercholesterolemia. Using RT-PCR analysis, bsh2, bsh3 and bsh4 were upregulated by bile salts in a dose-dependent manner. All three bsh genes had high similarity with those of other Lactobacillus strains. All three recombinant BSHs had high activities for the hydrolysis of glycodeoxycholic acids and taurodeoxycholic acids.
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