Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 9, Pages 1387-1394Publisher
SPRINGER
DOI: 10.1007/s10529-013-1218-2
Keywords
Animal cell culture; Flexible interfacial area; Liquid/liquid culture system; Multiphase bioreactors; Perfluorochemical (perfluorocarbon)
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Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O-2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect.
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