Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 10, Pages 1541-1549Publisher
SPRINGER
DOI: 10.1007/s10529-013-1249-8
Keywords
Algorithm; Computational intelligence; Polymerase chain reaction; Primer design
Categories
Funding
- National Science Council in Taiwan [NSC98-2221-E-151-040, NSC98-2622-E-151-001-CC2, NSC98-2622-E-151-024-CC3, 101-2221-E-464-001]
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The design of primers has a major impact on the success of PCR in relation to the specificity and yield of the amplified product. Here, we introduce the applications of PCR as well as the definition and characteristics for PCR primer design. Recent primer design tools based on Primer3, along with several computational intelligence-based primer design methods which have been applied in primer design, are also reviewed. In addition, characteristics of population-based methods used in primer design are discussed in detail.
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