Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 12, Pages 2137-2145Publisher
SPRINGER
DOI: 10.1007/s10529-013-1317-0
Keywords
Escherichia coli; Glucose-6-phosphate dehydrogenase; Lycopene; MEP pathway
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Funding
- National Basic Research Program of China (973 Program) [2012CB721101]
- National Special Fund for State Key Laboratory of Bioreactor Engineering [2060204]
- Shanghai Leading Academic Discipline Project [B505]
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Genetic manipulation was undertaken in order to understand the mechanism involved in the heterologous synthesis of lycopene in Escherichia coli. Knockout of the central carbon metabolic gene zwf (glucose-6-phosphate dehydrogenase) resulted in the enhancement of lycopene production (above 130 % relative to control). The amplification and overexpression of rate-limiting steps encoded by idi (isopentenyl diphosphate isomerase), dxs (1-deoxyxylulose-5-phosphate synthase) and ispDF (4-diphosphocytidyl-2C-methyl-d-erythritol synthase and 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase) genes improved lycopene synthesis from 0.89 to 5.39 mg g(-1) DCW. The combination of central metabolic genes knockout with the amplification of MEP pathway genes yielded best amounts of lycopene (6.85-7.55 mg g(-1) DCW). Transcript profiling revealed that idi and dxs were up-regulated in the zwf knock-out strain, providing a plausible explanation for the increase in lycopene yield observed in this strain. An increase in precursor availability might also have contributed to the improved lycopene production.
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