Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 5, Pages 685-688Publisher
SPRINGER
DOI: 10.1007/s10529-012-1131-0
Keywords
Nitrilase; Malononitrile; Enantioselective hydrolysis; Rhodococcus
Categories
Ask authors/readers for more resources
The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form (S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized conditions, 80 g (S)-CMPA l(-1) was produced with a molar yield of 97 % at 30 A degrees C after a 24 h without any by-products.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
