Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 9, Pages 1405-1409Publisher
SPRINGER
DOI: 10.1007/s10529-013-1224-4
Keywords
Biomass; Cellobiose; Miscanthus; Mono-saccharides; 1-Phenyl-3-methyl-5-pyrazolone; Xylo-oligosaccharides
Categories
Funding
- National Natural Science Foundation of China [31170067]
- National Basic Research Program of China (973 Program) [2010CB732201]
- Fundamental Research Funds for the Central Universities [201112G026]
Ask authors/readers for more resources
HPLC using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyse mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass. PMP derivatives, including those of mannose, rhamnose, cellobiose, glucose, xylose and arabinose, were separated within 14 min with detection at 254 nm. The method was also suitable for xylo-oligosaccharides (XOS): PMP derivatives of xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose were well separated under the same conditions. The method was used to determine the mono-saccharide composition of Miscanthus and evaluate the production of XOS from enzymatic hydrolysis of crude xylan.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
