Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 6, Pages 909-914Publisher
SPRINGER
DOI: 10.1007/s10529-013-1154-1
Keywords
Aptamer; Exponential enrichment; Systematic evolution of ligands; Vibrio alginolyticus
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Funding
- Scientific and Technological Project from the Education Department of Fujian Province [JB10096]
- Science Foundation of Jimei University [ZQ2011002]
- Foundation for Innovative Research Team of Jimei University [2010A004]
- Innovative Experimental Project for Students in Universities of Fujian Province
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Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 +/- A 9.2 nM. Using these aptamers and PCR, V. alginolyticus could be detected at 100 cells/ml. Sequencing of the final pool of aptamers revealed that some sequences, termed high-frequency aptamers, appeared more than once; these may be of practical application. All sequences obtained were divided into nine families according to their homology tree, some conserved sequences were also found in each of the six families. One sequence was found in significant proportions of the aptamers, suggesting that this conserved sequence might be important for forming the three-dimensional aptamer structure.
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