Journal
BIOTECHNOLOGY LETTERS
Volume 35, Issue 1, Pages 1-10Publisher
SPRINGER
DOI: 10.1007/s10529-012-0917-4
Keywords
Apoptosis; HepG2; Morchella esculenta; Polysaccharide; ROS generation
Categories
Funding
- Foundation for Key Research Program of Education Department of Anhui Province, China [KJ2009A172, KJ2011A017]
- National Natural Science Foundation of China [30800194]
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A novel polysaccharide, MEP-II, isolated from the fermentation broth of Morchella esculenta inhibited the proliferation of human hepatoma cell line (HepG2) through an apoptotic pathway. After HepG2 cells were treated with 150-600 mu g MEP-II/ml, typical apoptotic characteristics including externalization of phosphatidylserine residues on the cell surface, nuclear fragmentation, chromatin condensation and cytoplasm shrinkage were observed. Furthermore, reactive oxygen species (ROS) burst and the collapse of mitochondrial membrane potential (Delta psi m) also occurred in HepG2 cells after incubation of 150-600 mu g MEP-II/ml. The antioxidant, 1 mM N-acetyl-l-cysteine inhibited MEP-II-induced apoptosis, suggesting that ROS are the key mediators for MEP-II-induced apoptosis. MEP-II is therefore a potential anti-tumor agent that induces apoptosis of HepG2 cells through ROS generation.
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