Journal
BIOTECHNOLOGY LETTERS
Volume 34, Issue 7, Pages 1307-1313Publisher
SPRINGER
DOI: 10.1007/s10529-012-0902-y
Keywords
Immobilization; Response surface methodology; SiO2; Xylanase; Xylooligosaccharide
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Funding
- Converging Research Center through National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2011-50210]
- Ministry of Education, Science and Technology, Republic of Korea
- KU Brain Pool of Konkuk University
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Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k (cat) value (4,261 s(-1)) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.
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