Journal
BIOTECHNOLOGY LETTERS
Volume 34, Issue 8, Pages 1475-1482Publisher
SPRINGER
DOI: 10.1007/s10529-012-0916-5
Keywords
Biofuels; Cellulase; Enzyme recycle; Lignocellulose; Precipitation
Categories
Funding
- project Demonstrating Industrial scale second generation bioethaol production-Kalundborg Cellulosic Ethanol Plant under the EU FP7 framework program
- project Development of improved second generation (2G) bioethanol technology to prepare for commercialization under the Danish Energy Technology and Demonstration Programme (EUDP)
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Comparative studies between commercial Trichoderma reesei cellulase preparations show that, depending on the preparation and loading, total protein precipitation can be as high as 30 % under standard hydrolysis conditions used for lignocellulosic materials. ATR-IR and SDS-PAGE data verify precipitates are protein-based and contain key cell wall hydrolyzing enzymes. Precipitation increased considerably with incubation temperature; roughly 50-150 % increase from 40 to 50 A degrees C and 800 % greater at 60 A degrees C. All of the reported protein losses translated into significant, and often drastic, losses in activity on related 4-nitrophenyl substrates. In addition, supplementation with the non-ionic surfactant PEG 6,000 decreased precipitation up to 80 % in 24 h precipitation levels. Protein precipitation is potentially substantial during enzymatic hydrolysis of lignocelluloses and should be accounted for during lignocellulose conversion process design, particularly when enzyme recycling is considered.
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