Journal
BIOTECHNOLOGY LETTERS
Volume 34, Issue 4, Pages 717-720Publisher
SPRINGER
DOI: 10.1007/s10529-011-0827-x
Keywords
Deoxyviolacein; Escherichia coli; Janthinobacterium lividum; Violacein
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Funding
- German Academic Exchange Service [A-08-71658]
- Max-Buchner Foundation [2924]
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Violacein and deoxyviolacein display a broad range of interesting biological properties but their production is rarely distinguished due to the lack of suitable analytical methods. An HPLC method has been developed for the separation and quantification of violacein and deoxyviolacein and can determine the content of both molecules in microbial cultures. A comparison of different production microorganisms, including recombinant Escherichia coli and the natural producer Janthinobacterium lividum, revealed that the formation of violacein and deoxyviolacein is strain-specific but showed significant variation during growth although the ratio between the two compounds remained constant.
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