Journal
BIOTECHNOLOGY LETTERS
Volume 34, Issue 3, Pages 407-415Publisher
SPRINGER
DOI: 10.1007/s10529-011-0785-3
Keywords
Agrobacterium; Plant secondary metabolism; Ri Plasmid; Rol genes; T-DNA; Virulence proteins
Categories
Funding
- DBT
- UGC
- CSIR
- BTISNet SubDIC [BT/BI/04/065/04]
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Agrobacterium rhizogenes is a natural plant genetic engineer. It is a gram-negative soil bacterium that induces hairy root formation. Success has been obtained in exploring the molecular mechanisms of transferred DNA (T-DNA) transfer, interaction with host plant proteins, plant defense signaling and integration to plant genome for successful plant genetic transformation. T-DNA and corresponding expression of rol genes alter morphology and plant host secondary metabolism. During transformation, there is a differential loss of a few T-DNA genes. Loss of a few ORFs drastically affect the growth and morphological patterns of hairy roots, expression pattern of biosynthetic pathway genes and accumulation of specific secondary metabolites.
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