Journal
BIOTECHNOLOGY LETTERS
Volume 34, Issue 3, Pages 527-531Publisher
SPRINGER
DOI: 10.1007/s10529-011-0794-2
Keywords
Escherichia coli; Metabolic engineering; RyhB; sRNA
Categories
Funding
- National Natural Science Foundation of China [31070092]
- National Basic Research Program of China [2011CB707405]
- Key Scientific and Technological Program of Shandong province [2010GSF10202]
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Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCDAB genes. Acetate was also increased indicating that RyhB had a comprehensive influence on glucose central metabolism. RyhB might therefore be useful for metabolic engineering of E. coli.
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