Journal
BIOTECHNOLOGY LETTERS
Volume 33, Issue 9, Pages 1837-1840Publisher
SPRINGER
DOI: 10.1007/s10529-011-0631-7
Keywords
Bacillus licheniformis; Glutamate; Glutamate racemase; Poly-gamma-glutamic acid
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Funding
- Program for New Century Excellent Talents in University of China [NCET-07-0341]
- National High-Tech Research and Development Program of China [2008AA10Z317]
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The glr gene, which encodes glutamate racemase involved in the conversion of l-glutamic acid to its D-isomer, was cloned and expressed in Bacillus licheniformis WX-02. Overexpression of the glr gene not only increased the production of poly-gamma-glutamic acid (gamma-PGA) by 22.5% but also increased the proportion of d-glutamate in gamma-PGA from 77 to 85%. The activity of glutamate racemase was higher than in the original strain throughout cultivation. This is the first report that overexpression of the glr gene could enhance the l- and d-glutamate conversion in B. licheniformis WX-02 and increase the proportion of d-glutamate in gamma-PGA and the yield of gamma-PGA.
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