4.4 Article

Indigo biosynthesis by Comamonas sp MQ

Journal

BIOTECHNOLOGY LETTERS
Volume 34, Issue 2, Pages 353-357

Publisher

SPRINGER
DOI: 10.1007/s10529-011-0778-2

Keywords

Comamonas sp.; Indigo; Indole; Naphthalene

Funding

  1. National Natural Science Foundation of China [21176040, 51078054, 20923006]
  2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology [QAK201009]

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An indigo-producing strain was isolated from activated sludge and identified as Comamonas sp. based on 16S rRNA analysis. It produced indigo at 26.5 mg/l with a conversion of indole to indigo of 47%. Indole at 50 mg/l plus 200 mg naphthalene/l gave 32.2 mg indigo/l with a 58% conversion. A pathway for indigo formation is proposed. This is the first study of indigo biosynthesis by Comamonas sp.

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