Journal
BIOTECHNOLOGY LETTERS
Volume 33, Issue 6, Pages 1233-1238Publisher
SPRINGER
DOI: 10.1007/s10529-011-0537-4
Keywords
Giemsa C-banding; Partial amphiploid; Psathyrostachys huashanica; Stripe rust; Wheat
Categories
Funding
- National Transgenic Major Program [2008zx08009-001]
- National Natural Science Foundation of China [30870154, 30901052, 30900087]
- Bureau of Science and Technology
- Bureau of Education of Sichuan Province, China
Ask authors/readers for more resources
Two partial amphiploid lines, B113 (32 plants) and B21 (13 plants), derived from a wheat-Psathyrostachys huashanica intergeneric cross were characterized by Giemsa C-banding and SDS-PAGE and evaluated for stripe rust resistance. All 15 partial amphiploid plants were aneuploids with either 50 (8 plants), 51 (6 plants) or 54 (1 plant) chromosomes. Some showed regular meiosis and all the P. huashanica chromosomes were included, although not in a single plant. Of 45 plants 34 showed specific bands on SDS-PAGE representing high molecular weight glutenin subunit (HMW-GS) and 41 had bands representing P. huashanica low molecular weight glutenin subunit (LMW-GS), including two new subunits. All 45 plants were highly resistant (10) or immune (35) to stripe rust mixed races CYR-30, CYR-31, Shuiyuan 7 and Shuiyuan 14. These amphiploid plants could be useful germplasm for enhancing stripe rust resistance and might improve wheat grain quality.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available