Journal
BIOTECHNOLOGY LETTERS
Volume 33, Issue 12, Pages 2373-2377Publisher
SPRINGER
DOI: 10.1007/s10529-011-0727-0
Keywords
Aqueous two phase systems; Hematopoietic stem/progenitor cells; Human cell separation; Immunoaffinity partitioning
Categories
Funding
- Fundacao para a Ciencia e Tecnologia - FCT [PTDC/EQU-EPR/104663/2008, 3386/2010, SFRH/BD/38719/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/38719/2007, PTDC/EQU-EPR/104663/2008] Funding Source: FCT
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A novel cell separation process based on immunoaffinity aqueous two phase systems is presented to isolate and purify CD34(+) stem/progenitor cells directly from the whole umbilical cord blood (UCB). A system, composed of polyethylene glycol and dextran, was evaluated for the selective recovery of CD34(+) cells from UCB. A monoclonal antibody against the CD34 surface antigen was used for the direct partitioning of CD34(+) cells in UCB to the PEG-rich phase. The initial population of CD34(+) cells (0.2% of the initial sample) was enriched to values up to 42% in a single partitioning step, while the majority of contaminant cells were partitioned to the dextran-rich phase (1.37 x 10(-2) < K(P) < 2.76 x 10(-2)). This novel selection method allowed a recovery yield of 95% of CD34(+) cells with a purification factor of 245 and is expected to pave a new way to purify hematopoietic stem/progenitor cells for use in a variety of clinical settings.
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