Journal
BIOTECHNOLOGY LETTERS
Volume 33, Issue 8, Pages 1615-1619Publisher
SPRINGER
DOI: 10.1007/s10529-011-0596-6
Keywords
Chelex-100; Chlorella; Colony polymerase chain reaction; Genetic screening; Microalgae
Categories
Funding
- Chinese Natural Science Foundation For Distinguished Group [50621063]
- Central South University [2010bsxt05]
Ask authors/readers for more resources
A colony PCR technique was applied for both genomic and chloroplast DNA in the green microalgae Chlorella. Of five different lysis buffers, Chelex-100 was superior for DNA extraction, PCR and DNA storage. It also was insensitive to variations in cell density. The conditions established for an improved PCR formulation are applicable for screening of genetically-engineered transformants as well as bioprospecting of natural microalgal isolates. Besides multiple Chlorella species, we also demonstrate the efficacy of Chelex-100 for colony PCR with a number of other microalgal strains, including Chlamydomonas reinhardtii, Dunaliella salina, Nannochloropsis sp., Coccomyxa sp., and Thalassiosira pseudonana.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available