Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 5, Pages 661-667Publisher
SPRINGER
DOI: 10.1007/s10529-010-0209-9
Keywords
Furfural; Hexose; 5-Hydroxymethyl furfural; NADPH; Pentose; Transhydrogenase
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Funding
- U.S. Department of Energy [DE-FG36-08GO88142, DE-FC36-GO17058]
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The ability of a biocatalyst to tolerate furan inhibitors present in hemicellulose hydrolysates is important for the production of renewable chemicals. This study shows EMFR9, a furfural-tolerant mutant of ethanologenic E. coli LY180, has also acquired tolerance to 5-hydroxymethyl furfural (5-HMF). The mechanism of action of 5-HMF and furfural appear similar. Furan tolerance results primarily from lower expression of yqhD and dkgA, two furan reductases with a low K-m for NADPH. Furan tolerance was also increased by adding plasmids encoding a NADPH/NADH transhydrogenase (pntAB). Together, these results support the hypothesis that the NADPH-dependent reduction of furans by YqhD and DkgA inhibits growth by competing with biosynthesis for this limiting cofactor.
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