Journal
ANTICANCER RESEARCH
Volume 37, Issue 8, Pages 4303-4310Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.11823
Keywords
Prostate cancer; PCA3; GRP78; long non-coding RNA; cancer-related genes
Categories
Funding
- National Natural Science Foundation of China [81402292, 81571490]
- Shandong Provincial Natural Science Foundation, China [ZR2014HQ068]
- Yantai Science and Technology Development Plan [2014WS004, 2015WS024, 2016WS002]
Ask authors/readers for more resources
Background/Aim: The human prostate cancer antigen 3 (PCA3) is a long non-coding RNA (lncRNA) commonly used as a diagnostic marker for prostate cancer (PCa). Herein we investigated the cellular function of PCA3 in PCa and its potential mechanism. Materials and Methods: PCA3 was overexpressed in a PC3 cell line (PC3(PCA+)) and cell proliferation, migration, invasion and apoptosis were compared to those of control cells (PC3(NC)). Differentially expressed proteins were identified by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry. Results: Overexpression of PCA3 significantly increased cell proliferation rate, migration and invasion, while inhibited apoptosis in PC3 cells. Three proteins were found down-regulated and 7 proteins up-regulated in PC3(PCA+) cells compared to PC3(NC) cells, including GRP78. Higher GRP78 was also found in PCa clinical specimens. Conclusion: This study confirmed that in PCa, PCA3 plays a pro-cancer role through promoting cell proliferation, migration and invasion while inhibiting cell apoptosis. This process might involve the up-regulation of GRP78.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available