Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 9, Pages 1287-1291Publisher
SPRINGER
DOI: 10.1007/s10529-010-0288-7
Keywords
Activation; Enzyme; Kinetics; Lipase; Michaelis-Menten; Pressure
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Funding
- University of Florida Institute of Food and Agricultural Sciences
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Lipase-catalyzed synthesis of isoamyl acetate in hexane at 10-250 MPa at 80A degrees C and 1-100 MPa at 40A degrees C resulted in activation volumes of -12.9 +/- A 1.7 and -21.6 +/- A 2.9 cm(3) mol(-1), respectively. Increasing pressure from 10 to 200 MPa resulted in approximately 10-fold increase in V (max) at both 40 and 80A degrees C. Pressure increased the K (m) from 2.4 +/- A 0.004 to 38 +/- A 0.78 mM at 40A degrees C. In contrast, at 80A degrees C the pressure did not affect the K (m).
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