Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 12, Pages 1877-1881Publisher
SPRINGER
DOI: 10.1007/s10529-010-0364-z
Keywords
Glutamine; gamma-Glutamylglutamine; gamma-Glutamyltranspeptidase; Transpeptidation
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [21380059]
- Grants-in-Aid for Scientific Research [21380059] Funding Source: KAKEN
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The optimal reaction conditions for the synthesis of gamma-glutamylglutamine using gamma-glutamyltranspeptidase from Escherichia coli were determined. The maximum yield of gamma-glutamylglutamine ( 110 mM) was obtained using 250 mM L-glutamine and 1.1 U gamma-glutamyltranspeptidase/ml at pH 10.5 and at 37 degrees C for 7 h; the conversion of glutamine to gamma-glutamylglutamine was 88%.
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