Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 6, Pages 817-821Publisher
SPRINGER
DOI: 10.1007/s10529-010-0217-9
Keywords
Activity; Fe3O4/SiO2 magnetic nanoparticles; Glucose oxidase; Immobilization; Stability
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Funding
- National Natural Science Foundation of China [60877048]
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Glucose oxidase (GOD) was covalently immobilized onto Fe3O4/SiO2 magnetic nanoparticles (FSMNs) using glutaraldehyde (GA). Optimal immobilization was at pH 6 with 3-aminopropyltriethoxysilane at 2% (v/v), GA at 3% (v/v) and 0.143 g GOD per g carrier. The activity of immobilized GOD was 4,570 U/g at pH 7 and 50A degrees C. The immobilized GOD retained 80% of its initial activity after 6 h at 45A degrees C while free enzyme retained only 20% activity. The immobilized GOD maintained 60% of its initial activity after 6 cycles of repeated use and retained 75% of its initial activity after 1 month at 4A degrees C whereas free enzymes retained 62% of its activity.
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