Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 12, Pages 1933-1937Publisher
SPRINGER
DOI: 10.1007/s10529-010-0379-5
Keywords
Hybridization; Influenza virus; Magnetic beads; Quantum dots
Categories
Funding
- National Key Scientific Program (973)-Nanoscience and Nanotechnology [2006CB933100]
- National Major Project in Infectious Diseases [2009ZX10004-107]
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A new method to detect H5 influenza virus using quantum dots (QDs) and magnetic beads (MBs) is described. QDs conjugated with oligonucleotide probes were used to produce fluorescent signals and MBs, that were also conjugated with probes, were used to isolate and concentrate the signals. Target viral RNAs led to a sandwich hybridization between the functionalized QDs and MBs. One-step hybridization facilitated the subtype determination. As little as 0.1 ng viral RNA could be detected.
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