Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 9, Pages 1279-1285Publisher
SPRINGER
DOI: 10.1007/s10529-010-0286-9
Keywords
Antifungal activity; Plant protection; Phenazine; Pseudomonas fluorescens; Pyoluteorin
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Funding
- Ministry of Higher Education and Scientific Research
- Centre Francais pour l'Accueil et les Echanges Internationaux
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Pseudomonas fluorescens Pf1TZ was inhibitory in vitro to a number of phytopathogenic fungi and could protect vine plantlets against Botrytis cinerea. Total protection was reached after 3 weeks of bacterial inoculation. The endophytism of Pf1TZ was confirmed by confocal microscopy using its inherent fluorescence. The molecular characterization of Pf1TZ indicated the presence of genes from clusters encoding pyoluteorin and phenazine. The chromatographic purification and LC-MS(n) analysis revealed that the most active one has a molecular mass of 504 Da.
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