Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 10, Pages 1481-1486Publisher
SPRINGER
DOI: 10.1007/s10529-010-0315-8
Keywords
Biosynthetic pathway; Cell-free protein synthesis; Glucokinase; N-acetylglucosamine-phosphate mutase; N-acetylglucosamine-1-phosphate uridyltransferase; Uridine 5 '-diphospho N-acetylglucosamine
Categories
Funding
- National Natural Science Foundation of China [20736008]
- Ministry of Science and Technology [2007AA021702, 2009CB918601]
- Natural Science Foundation of Zhejiang [R4090041]
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A functional glucokinase (YqgR), N-acetylglucosamine-phosphate mutase (Agm1) and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) were synthesized simultaneously in an Escherichia coli cell-free system. These three enzymes were coupled to reconstruct a biosynthetic pathway which could produce UDP-N-acetylglucosamine with N-acetylglucosamine as the substrate.
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