Journal
BIOTECHNOLOGY LETTERS
Volume 31, Issue 11, Pages 1783-1788Publisher
SPRINGER
DOI: 10.1007/s10529-009-0079-1
Keywords
Leuconostoc citreum; Heterologous protein; alpha-Amylase; Segregational stability; pLeuCM
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Funding
- Laboratory of Strategic Technology from MOCIE, Korea
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To develop a gene expression system for Leuconostoc genus, construction of expression vector and expression of a heterologus protein in Leuconostoc was performed. alpha-Amylase gene from Lactobacillus amylovorus was cloned into a Leuconostoc cloning vector, pLeuCM, with its own signal peptide. pLeuCMamy was introduced into Leuconostoc citreum CB2567 and a successful expression of alpha-amy gene was confirmed by enzyme activity assays. About 90% of alpha-amylase activity was detected in the culture broth, revealing most of expressed alpha-amylase was secreted out cells. The signal sequence of alpha-amy gene is a good candidate for the secretion of heterologous protein by using Leuconostoc host-vector system.
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