Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 4, Pages 517-520Publisher
SPRINGER
DOI: 10.1007/s10529-009-0187-y
Keywords
Cellobiose utilization; Lactobacillus lactis; D-Lactic acid production; Mutant; Sucrose (cane sugar)
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Funding
- NMITLI Division, Council of Scientific and Industrial Research (CSIR), New Delhi, India
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Three mutants, isolated by repeated UV mutagenesis of Lactobacillus lactis NCIM 2368, produced increased d-lactic acid concentrations. These mutants were compared with the wild type using 100 g hydrolyzed cane sugar/l in the fermentation medium. One mutant, RM2-24, produced 81 g lactic acid/l which was over three times that of the wild type. The highest d-lactic acid (110 g/l) in batch fermentation was obtained with 150 g cane sugar/l with a 73% lactic acid yield. The mutant utilizes cellobiose efficiently, converting it into d-lactic acid suggesting the presence of cellobiase. Thus, this strain could be used to obtain d-lactic acid from cellulosic materials that are pre-hydrolyzed with cellulase.
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