Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 1, Pages 59-66Publisher
SPRINGER
DOI: 10.1007/s10529-009-0123-1
Keywords
Biodegradability; Carboxymethylation; Chitosan; Cross-linking; Peripheral nerve regeneration
Categories
Funding
- National Basic Research Program of China [2005CB623905]
- Tsinghua-Yue-Yuen Medical Science Fund
- National Natural Science Foundation of China [30670528, 30700848, 30772443]
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A successful nerve regeneration process was achieved with nerve repair tubes made up of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linked carboxymethyl chitosan (CM-chitosan) with improved biodegradability. Chitosan has a very slow degradation rate, while the EDC cross-linked CM-chitosan tubes degraded to 30% of original weight during 8 weeks of incubation in lysozyme solution. In vitro cell culture indicated that the CM-chitosan films presented no cytotoxicity to Schwann cells. From in vivo studies using a 10 mm rat sciatic nerve defect model investigated by histomorphometry analysis, the average diameter of the fibers and the average thickness of myelin sheath in the CM-chitosan tubes were 3.7 +/- A 0.33 and 0.33 +/- A 0.04 mu m, respectively, which demonstrated equivalence to nerve autografts (the current gold standard); furthermore, the average fiber density in the CM-chitosan tubes was 20.5 x 10(3)/mm(2), which was similar to that of autografts (21 x 10(3)/mm(2)) and significantly higher than that of common chitosan tubes (15.3 x 10(3)/mm(2)).
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