Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 4, Pages 527-531Publisher
SPRINGER
DOI: 10.1007/s10529-009-0193-0
Keywords
Central carbon metabolism; Glucose 6-phosphate; Rate-limiting step; Xanthomonas oryzae pv. oryzae; Xanthan
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Funding
- Rural Development Administration, Republic of Korea [20090101 060 026 001 02 00]
- Sangji University Research Fund
- Gangwon Technopark
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Two genes involved in central carbon metabolism were inactivated to modulate intracellular glucose 6-phosphate and to evaluate its effects on xanthan production in wild-type Xanthomonas oryzae pv. oryzae. Upon the inactivation of the phosphogluconate dehydratase gene (edd), intracellular glucose 6-phosphate increased from 0.05 to 1.17 mmol/g (dry cell wt). This was accompanied by increased xanthan production of up to 2.55 g/l (culture medium). In contrast, inactivation of 6-phosphogluconate dehydrogenase gene (gndA) did not influence intracellular glucose 6-phosphate nor xanthan production. The intracellular availability of glucose 6-phosphate is proposed as a rate-limiting factor in xanthan production, and it may be possible to increases production of xanthan by modulating the activities of enzymes in central carbon metabolism.
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