Journal
BIOTECHNOLOGY LETTERS
Volume 32, Issue 4, Pages 565-570Publisher
SPRINGER
DOI: 10.1007/s10529-009-0180-5
Keywords
Allergens; Parietaria judaica; Immunoproteome; Pollen
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Funding
- ARPA Sicilia
- Universita di Palermo (International Cooperation)
- ex 60% MURST
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Parietaria judaica pollen is a common cause of airway allergic disease in the Mediterranean area. Proteome analysis of mature Parietaria judaica pollen by two-dimensional gel electrophoresis (2-DE) and mass spectrometry has established the first reference proteome map of this weed. Proteins involved in a variety of cellular functions as well as the occurrence of allergens were detected. By using 2-DE and immunoblotting with sera from Parietaria judaica allergic patients we obtained a more detailed characterization of Parietaria judaica allergen profile so to improve our comprehension of the pathogenesis of pollen-induced allergic reaction.
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