Journal
BIOTECHNOLOGY LETTERS
Volume 31, Issue 7, Pages 1005-1010Publisher
SPRINGER
DOI: 10.1007/s10529-009-9958-8
Keywords
Antifungal activity; beta-1,3-Glucanase; Expression; Rhizoctonia cerealis; Triticum aestivum
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Funding
- National Hi-Tech'' project [2006AA10A104]
- National Key Scie-Tech'' project [2008ZX08002-001]
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A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
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