Journal
BIOTECHNOLOGY LETTERS
Volume 31, Issue 11, Pages 1775-1782Publisher
SPRINGER
DOI: 10.1007/s10529-009-0075-5
Keywords
G protein-coupled receptors; Heterologous protein expression; Sigma factor; Stress response
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Funding
- Princeton University
- Lidow Senior Thesis
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The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.
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