Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta

A laccase was purified from Trametes hirsuta. This laccase was classified as a white or yellow laccase. pH 2.4 was optimal for the oxidation of ABTS and pH 2.5 for DMP. DMP oxidation was optimal at 85A degrees C. The half-life of this laccase was 70 min at 75A degrees C, and 5 h at 65A degrees C. Non-phenolic dyes, such as Methyl Red, were oxidized by purified laccase without mediators. The enzyme was not inhibited by Cu2+, Mn2+, or EDTA. These are atypical laccase characteristics that make it a good candidate for theoretical and applied research.