Journal
BIOTECHNOLOGY LETTERS
Volume 31, Issue 6, Pages 877-881Publisher
SPRINGER
DOI: 10.1007/s10529-009-9941-4
Keywords
Escherichia coli; Gene expression; Levansucrase gene; Rahnella aquatilis; Promoter
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Funding
- Korean Research Foundation
- KRIBB
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The levansucrase gene (lsrA) from Rahnella aquatilis was strongly expressed in a constitutive manner in Escherichia coli when cloned into a pBluescript KS-based pRL1CP plasmid vector. The native promoter upstream of lsrA and the lacZ promoter cooperatively enhanced the expression of lsrA to a level that was comparable to that of the T7 promoter, which is used in commercial pET expression vector system. A putative rho-independent transcription termination signal downstream of lsrA was crucial for gene expression. This plasmid vector also proved to be applicable for efficient expression of other foreign genes in E. coli.
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